Novel Clade 1: Pseudopirsonia, AuranticordisDavid Bass, Tom Cavalier-Smith, and Chitchai Chantangsi
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The cercozoan Novel Clade 1 was first established by Bass & Cavalier-Smith (2004) on the basis of molecular phylogenetic study inferred from SSU rDNA sequences. This clade originally contained only one described taxon, Pseudopirsonia mucosa, and a few other sequences derived from environmental studies of marine habitats. Subsequent study by Chantangsi et al. (2008) discovered another new member of this clade, namely Auranticordis quadriverberis (Figure 1). Other gliding zooflagellates also probably belong here.
Pseudopirsonia was first discovered in a study of parasitoid nanoflagellates–that is, very small eukaryotes that parasitize other protists, often phytoplankton (Kühn et al. 1996). This genus was originally described as a member of Pirsonia, which are heterokont parasitoids of planktonic diatoms, most closely related to Hyphochytrium (Kühn et al. 1996; Schnepf et al. 1990). However, phylogenetic analyses show that Pseudopirsonia is cercozoan and differs from true Pirsonia in morphology, movement, and feeding behaviour. However, the life cycles of the two genera show many similarities, and both include formation of conspicuous trophosomes and auxosomes (Kühn et al. 2004). Auranticordis is an unusual orange coloured marine free-living gliding zooflagellate with four posterior flagella (Chantangsi et al. 2008).
The group is too little studied for their key unifying structural characters to be determined, but known species have either two or four flagella inserted ventrally (Chantangsi et al. 2008; Kühn et al. 1996).
Cercozoan members of the Novel Clade 1 inhabitat aquatic marine environments ranging from a cold (6°C) to temperate (15°C) areas (Kühn et al. 1996). Pseudopirsonia and Auranticordis were originally isolated from North Sea, Wadden Sea near List (Sylt, Germany) and Spanish Banks, Vancouver (BC, Canada), respectively (Chantangsi et al. 2008; Kühn et al. 1996).
Life Cycle and Development
Life cycle and development were studied only in Pseudopirsonia. Briefly, Pseudopirsonia parasitizes diatoms by intruding its pseudopods into the diatoms’ frustules and phagocytoses portions of the diatom protoplast. The pseudopodial part of the organism located inside the diatom cell differentiates into the trophosome. Digested materials and nutrients are then transported into the body of the flagellate, now called auxosome, attaching outside of the diatom frustule. The auxosome develops and divides longitudinally, forming offspring as long as trophosomes continue to phagocytose. This developing cell forms a globular, morula-like group in various arrangements usually coated with mucilage. (Kühn et al. 1996, 2004).
The phylogeny in Kühn et al. (2004) shows Pseudopirsonia as a close relative of a Cercomonas strain (GenBank Accession No. AF277495). However, this sequence is mis-labelled and is not a cercomonad. The cercozoan trees in Bass & Cavalier-Smith (2004), Bass et al. (2005), and Cavalier-Smith et al. (2008) show that Pseudopirsonia belongs to cercozoan Novel Clade 1 (Bass & Cavalier-Smith 2004), which groups in an indeterminate position near spongomonads and/or imbricates. Other members of novel clade 1 are only known from environmental sequences, all from marine (coastal) water columns.
A study by Chantangsi et al. (2008) shows close phylogenetic relationships among Auranticordis, Pseudopirsonia, and two other sequences derived from unidentified taxa (GenBank Accession No. AB252755 and AB275058), forming the Auranticordida clade. Members of this clade are all from saline habitats and also share a derived molecular character within the context of 160 cercozoan sequences covering representatives from all known cercozoan subclades: namely, the substitution of cytosine (C) for thymine (T) at position 324 (with reference to the complete SSU rDNA sequence of Cercomonas sp.; GenBank accession No. AF411266, culture ATCC PRA-21) in Helix 12, based on the predicted secondary structure of the SSU rRNA gene in Palmaria palmata (Wuyts et al. 2000).
Bass D, Cavalier-Smith T (2004) Phylum-specific environmental DNA analysis reveals remarkably high global biodiversity of Cercozoa (Protozoa). Int J Syst Evol Microbiol 54: 2393-2404
Bass D, Moreira D, López-García P, Polet S, Chao EE, von der Heyden S, Pawlowski J, Cavalier-Smith T (2005) Polyubiquitin insertions and the phylogeny of Cercozoa and Rhizaria. Protist 156: 149-61
Cavalier-Smith T, Lewis R, Chao EE, Oates B, Bass D (2008) Morphology and phylogeny of Sainouron acronematica sp. n. and the ultrastructural unity of Cercozoa. Protist 159: 591-620
Chantangsi C, Esson HJ, Leander BS (2008) Morphology and molecular phylogeny of a marine interstitial tetraflagellate with putative endosymbionts: Auranticordis quadriverberis n. gen. et sp. (Cercozoa). BMC Microbiol 8: 123
Kühn SF, Drebes G, Schnepf E (1996) Five new species of the nanoflagellate Pirsonia in the German Bight, North Sea, feeding on planktonic diatoms. Helgoländer Meeresunters 50: 205–222
Kühn SF, Medlin LK, Eller G (2004) Phylogenetic position of the parasitoid nanoflagellate Pirsonia inferred from nuclear-encoded small subunit ribosomal DNA and a description of Pseudopirsonia n. gen. and Pseudopirsonia mucosa (Drebes) comb. nov. Protist 155: 143-156
Schnepf E, Drebes G, Elbrächter M (1990) Pirsonia guinardiae, gen. et spec. nov.: A parasitic flagellate on the marine diatom Guinardia flaccida with an unusual mode of food uptake. Helgoländer Meeresunters 44: 275–293
Wuyts J, De Rijk P, Van de Peer Y, Pison G, Rousseeuw P, De Wachter R (2000) Comparative analysis of more than 3000 sequences reveals the existence of two pseudoknots in area V4 of eukaryotic small subunit ribosomal RNA. Nucleic Acids Res 28: 4698-4708
This page is being developed as part of the Tree of Life Web Project Protist Diversity Workshop, co-sponsored by the Canadian Institute for Advanced Research (CIFAR) program in Integrated Microbial Biodiversity and the Tula Foundation.
Correspondence regarding this page should be directed to David Bass at , Tom Cavalier-Smith at , and Chitchai Chantangsi at
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- First online 06 May 2009
- Content changed 06 May 2009
Citing this page:
Bass, David, Tom Cavalier-Smith, and Chitchai Chantangsi. 2009. Novel Clade 1: Pseudopirsonia, Auranticordis. Version 06 May 2009 (under construction). http://tolweb.org/Novel_Clade_1%3A_Pseudopirsonia%2C_Auranticordis/126325/2009.05.06 in The Tree of Life Web Project, http://tolweb.org/